The coating of a lipid bilayer membrane on a glass is very common in microfluidics to form a supported lipid bilayer membrane. In the experiment GUVs are produced and deposited onto the glass substrate. In this work we report how ionic solutions may affect the interaction between the GUV and the glass substrate. Experimentally we observed at least two scenarios of GUV-glass substrate interactions leading to different spreading dynamics. Theoretically we try to model how the concentration of ionic solutions may affect the vesicle-glass interaction, leading to different membrane tension distribution prior to vesicle rupture and spreading.