The decisive steps in connecting free probability, free noncommutative functions theory, and random matrix theory are due to Dan Voiculescu. In a breakthrough from the early nineties, he proved that, roughly speaking, large independent random matrices are asymptotically free as their size tends to infinity. This result opened the way for a series of revolutionary applications of random matrix theory to operator algebras, and, conversely, allowed a vast array of very powerful tools from noncommutative analysis to be deployed in the study of random matrices. Free noncommutative functions turned out to be the natural analytic transforms for the study of noncommutative distributions, as it was found out by Voiculescu in a series of four papers dating from the beginning of the 2000s. Since then, these tools have become, under various guises, a mainstay in the study of both local and global properties of random matrices, as well as in their applications to various problems in operator algebras. This workshop will focus on the most recent developments in these fields, and foster exchanges between specialists from random matrix theory, free noncommutative functions, and operator algebras.